Figure 5: Inhibition of macrophage and DC conversion to OC with miR-142-3p overexpression.

Human CD14+ monocytes were cultured in the presence of M-CSF or GM-CSF plus IL-4 for differentiation into macrophages and DC, respectively. Macrophage and DC were transfected at day 7 with miR-142-3p mimic, miR-142-3p inhibitor, or control mimic, and culture conditions were substituted for OC-differentiating conditions. (A) Representative images of macrophage and DC at day 7, at day 11 with transfection and OC-differentiating conditions, and day 11 negative controls (control mimic transfected, absence of OC-differentiating conditions). Cells were stained using MitoTracker (mitochondria, red). (B) Viability of macrophage-to-OC and DC-to-OC cultures from day 8 to day 11 assessed by MTS assay. (C) Collagen degradation of macrophage-to-OC and DC-to-OC cultures (untransfected) assayed at day 14. Data are presented as mean and SD, or representative images, from 3 biological donors. *p < 0.05 relative to untreated control cells. P values were calculated using the Student’s t-test.