Figure 4 | Scientific Reports

Figure 4

From: Three TaFAR genes function in the biosynthesis of primary alcohols and the response to abiotic stresses in Triticum aestivum

Figure 4

Heterologous expression of the three TaFARs in yeast mutant INVSur4#.

The yeast expressing empty vector control pYES3 (a) or vector harboring TaFAR2 (b), TaFAR3 (c) or TaFAR4 (d). Transgenic yeast cells were grown in stringent medium lacking trptophan and uracil. Primary alcohols were separated from other neutral lipids by TLC. In empty vector control, no primary alcohols were detected. In contrast, yeast harboring TaFAR2, TaFAR3 and TaFAR4 preferentially produced C18, C28 and C24 primary alcohols, respectively. All primary alcohols were also observed in wheat (cv. Chinese Spring, (e) leaf.

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