Figure 9

Effects of air humidity on epicuticular wax deposition.
Three-week old Chinese Spring seedlings grown under low (10–20% RH) and high (90–95% RH) humidity were used for subsequent analysis. (a) Relative expression analysis of TaFARs by qRT-PCR. H, high (90–95% RH) humidity; L, low (10–20% RH) humidity. (b,c) The cuticular wax composition and chain length distribution within compound classes. All values represent means of three independent parallel experiments. Prim. alcohols, primary alcohols. Error bars indicate SD and asterisks represent significant differences (t-test: * for P < 0.05; ** for P < 0.01). (d) Elevation of epicuticular wax crystals after low humidity treatment. Wax crystals on adaxial and abaxial surfaces of wheat leaves were detected by SEM at 10,000X magnification. Bars = 2 μm.