Figure 2
miR-127-5p inhibits OPN expression.
(A,B) Expression of miR-127-5p in chondrocytes after miR-127-5p mimics (A) or miR-127-5p inhibitor (B) transfection. (C,D) Protein abundance of OPN in the chondrocytes after miR-127-5p mimics (C) or miR-127-5p inhibitor (D) transfection. (E) The illustration of OPN wild- type/mutant 3′-UTRs containing the putative miR-127-5p binding sites. (F) The luciferase activity in chondrocytes co-transfected with the wild-type or mutant 3′-UTR reporter vector and the miR-127-5p mimics. (G) The luciferase activity in chondrocytes co-transfected with the wild-type or mutant 3′-UTR reporter vector and the miR-127-5p inhibitor. Data are representative of three independent experiments with 4-6 repeats in each time. OPN: osteopontin. Mi-C: miR-127-5p mimics control; I-C: miR-127-5p inhibitor control.
