Figure 2: Prorenin stimulated FN and α-SMA expression of NRK52E cells with different concentrations (a: 0, 10, 20, 40, 80, or 100 pmol/L for 24 hours) or the different treatment times (b: 100 pmol/L for 0, 6, 12, 24, 36, or 48 hours). | Scientific Reports

Figure 2: Prorenin stimulated FN and α-SMA expression of NRK52E cells with different concentrations (a: 0, 10, 20, 40, 80, or 100 pmol/L for 24 hours) or the different treatment times (b: 100 pmol/L for 0, 6, 12, 24, 36, or 48 hours).

From: Interaction between V-ATPase B2 and (Pro) renin Receptors in Promoting the progression of Renal Tubulointerstitial Fibrosis

Figure 2

(A) Real-time PCR analysis showed mRNA expression of α-SMA and FN. Values are means ± SD; n = 4. *P < 0.05 vs. control group (0 hour or 0 pmol/L). (B) Western blot analysis of protein expression of α-SMA and FN. β-actin is an internal control. (C) Graphic representation of the protein levels of α-SMA and FN at different time or concentration points, as indicated after normalization with β-actin. Values are means ± SD; n = 4. *P < 0.05 vs. control group (0 hour or 0 pmol/L). (D) Phase-contrast images (magnification ×100) were captured to analyze cell morphology of NRK52E cells in control or prorenin stimulated group (100 pmol/L for 48 h).

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