Figure 2
From: Baicalein pretreatment reduces liver ischemia/reperfusion injury via induction of autophagy in rats
Baicalein increases autophagy after I/R.
Rats were pretreated with either baicalein (100 mg/kg, IP) or vehicle (DMSO) 1 h prior to ischemia. (a) Western blot analysis of LC3B protein expression in the presence and absence of chloroquine (60 mg/kg). GAPDH was used as the loading control. (b) Densitometric analysis of LC3B-II expression. (c) Representative transmission electron micrographs showing autophagosomes in the ischemic lobes at 6 h of reperfusion. Autophagosomes are indicated by arrows. Scale bars 1 µm. (d) The numbers of autophagosomes were determined. The data are shown as the mean ± SD. n = 6 per group.
