Figure 8
From: Baicalein pretreatment reduces liver ischemia/reperfusion injury via induction of autophagy in rats
Inhibition of HO-1 prevents the baicalein-induced autophagy and protection after H/R in primary hepatocytes.
Hepatocytes were treated with SnPP (50 μM, 1 h) or HO-1 siRNA (50 nM, 48 h) prior to baicalein treatment (5 μM, 1 h) and followed by 6 h hypoxia and 2 h reoxygenation. (a) HO-1 protein expression was examined by western blot analysis. (b) Densitometric analysis of HO-1 expression. (c) Immunoblots indicating expression of HO-1 protein after treatment with HO-1 siRNA for 48 h. (d) Densitometric analysis. (e) Representative fluorescence micrographs display autophagy vacuoles (yellow: autophagosomes; red: autolysosomes) in hepatocytes with SnPP in the presence or absence of baicalein. (f) Representative fluorescence micrographs display autophagy vacuoles in hepatocytes with HO-1 siRNA in the presence or absence of baicalein. The scale bar represents 30 μm. (g) Quantification of autophagosomes and autolysosomes. (h) Quantification of cell viability and LDH release of primary hepatocytes. The experiment was performed in triplicates with similar results. The data are shown as the mean ± SD.
