Figure 3

LIBS-MPIO contrast agent specifically binds to activated platelets of pulmonary thromboembolism.

(a) Immunohistochemistry staining for platelet CD41 in thrombin-induced pulmonary embolism (PE) after injection of LIBS-MPIO or Control-MPIO. ↘ MPIO visible as small spheres on the surface of CD41⁺ cells. Bar indicates 10 μm. (b) Number of PE per left lung (left) after injection of human thrombin and LIBS-MPIO compared to thrombin and Control-MPIO Quantification of mean MPIO-binding per PE section (right) was performed by counting MPIO co-localized to CD41⁺ cells using light microscopy with 63x to 100x magnification. (n = 6–8 mice per group, p < 0.05; ns = not significant, Mann-Whitney U Test). (c) Correlation of PE grade with number of MPIOs bound to PE section after injection of LIBS-MPIO or Control-MPIO (n = 6 mice per group, p < 0.05, Pearson correlation). Data are shown as mean ± s.e.m.