Figure 1

cDCs and pDCs are generated at different sites.

Lymphoid organs were isolated from Fucci-Green mice and C57BL/6 mice. Cells were stained for surface markers. Gated cell populations were then analysed for expression of Geminin-GFP. (A) spleen cDCs and pDCs; (B) BM Siglec H⁺ cells. (C) Mean ± SEM of GFP⁺ DCs of spleen, LN and BM from 5 individual mice are shown for one of 3 independent experiments; all three experiments had similar results. (D) CD11b⁻Siglec H⁺ cells from BM and spleen of Fucci-Green mice were separated based on expression of CCR9 and CD4. The expression of Geminin-GFP by subsets was shown. More than 3 three experiments were performed.