Figure 2 | Scientific Reports

Figure 2

From: Plasmacytoid dendritic cells are short-lived: reappraising the influence of migration, genetic factors and activation on estimation of lifespan

Figure 2

Early BrdU labeling occurs in BM pDCs and spleen cDCs.

C57BL/6 mice were given an intraperitoneal injection of 0.1 mg BrdU and then fed with BrdU water (0.8 mg/mL). Spleens and BM were harvested at 1–4 days after BrdU exposure. Cells were stained for cell surface markers and then for intracellular BrdU. (A) Gating of pDCs and cDCs of spleen and BM. (B) Representative facs plots of BrdU labeling of gated spleen DC subsets. (C) Representative facs plots of BrdU labeling of BM pDCs. (D) Kinetics of BrdU labeling of DCs from spleen and BM. Two independent experiments were performed with similar results. (E) Estimation of migration time of pDC subsets, based on the data in (D).

Back to article page