Figure 5
pDCs of NZB mice survive longer than pDCs of C57BL/6 mice in vivo.
(A–D) Spleens and BM of age- (6–8 weeks old) and sex-matched C57BL/6 and NZB mice were harvested. Single cell suspension was stained for DC surface markers. Mean ± SEM of numbers and proportions of total and gated populations are shown for 13 C57BL/6 and 11 NZB mice pooled from 2 experiments; **P < 0.01, ***P < 0.001 by Student t test. (E) C57BL/6 and NZB mice were fed BrdU water. Organs were harvested at different days for evaluation of BrdU labeling of gated DC populations. (F) C57BL/6 mice and NZB mice were given a single dose of 5-FU intravenously. After 3 days, DC composition in spleen and BM was evaluated. Mean ± SEM of 3 mice from each strain are shown for one of three independent experiments. **P < 0.01 by Student t test.
