Figure 4: PTD1 enhances triple fusion K-L eradication of dynamic MRSA biofilms.
From: Triple-acting Lytic Enzyme Treatment of Drug-Resistant and Intracellular Staphylococcus aureus
(A) Confocal microscopy of biofilm with Live/Dead staining. Single 1 μm z-stack images in the middle of NRS382 (USA100) biofilms treated with 100 μg/ml (1.4 μM) triple fusion K-L, K-L-PTD1, (69 μM) vancomycin (Van) at a flow rate of 0.5 ml/min for 0, 60, or 120 minutes. Biofilms were pre-stained with the Live/Dead stain (see methods) and viewed with 20X magnification. (B) Bacterial viability. Analysis of bacterial viability in NRS382 dynamic biofilms based on mean fluorescent intensities of the Live/Dead viability stain when exposed to 100 μg/ml of PGH K-L (1.4 μM), K-L-PTD1 (1.4 μM), or vancomycin (69 μM) at a flow rate of 0.5 ml/min for 2 h and compared to PBS. Error bars represent SEM (N = 3) of three independent 100 × 100 pixel squares identically located in each biofilm z-stack. All values were found to be significantly different from PBS and each other using a two-tailed, unpaired, t-test; K-L vs. KL-PTD1 (p = 0.00064), K-L vs. vancomycin (p = 0.00036), and K-L-PTD1 vs. vancomycin (p = 0.000023), as indicated with an asterisk.
