Figure 5: Effect of misTTR on TTR fibrillogenesis.

(A) Time course of TTR fibril formation monitored by turbidity (3.6μM, 37 °C, pH 4.5). Substoichiometric amounts of misTTR were added at time 0 and after 30 hours to determine effect on TTR fibrillization process. (B) Dose dependent inhibition of TTR fibril formation by misTTR after 72 h. ThT emission peak was integrated from 475 nm to 495 nm and plotted versus antibody concentration (IC50 = 9.08 ± 0.32 nM).