Figure 2
The AutA and AutR regulated the expression of UpaB in DE205B in vitro.
(A) Quantification of the UpaB expression among DE205B mutants and complemented strains. The transcription of upaB gene among DE205B variants was determined by qRT-PCR. Three replicates were normalized to the transcription of housekeeping gene dnaE and the fold change of upaB transcription among DE205B variants relative to the DE205B was calculated with the ΔΔCT method. (B) The β-galactosidase activity of each upaB::lacZ transcriptional fusion among DE205B variants was assayed by the Miller assay. The upaB transcriptional change was indirectly evaluated by the determination of β-galactosidase activity (in Miller units) among DE205B variants. Statistical significance analysis was performed using two-way ANOVA (*P < 0.05).
