Figure 5
Promoters with distinct normal cell and persister gene expression patterns have a subpopulation that continues to synthesize protein in stationary phase.
(a) Growth curves of reporter strains show that reporter strains reach stationary phase by ~8 h. Data are averages of 3 biological replicates; error bars portray s.e.m. (b–f) Promoters with distinct normal cell and persister gene expression patterns contain a subpopulation which continues to actively synthesize protein in stationary phase, as demonstrated by increasing fluorescence between hours 8–16. (g,h) Promoters not demonstrating distinct normal cell and persister gene expression patterns exhibit constant low fluorescence throughout stationary phase. Histograms are representative of 3 biological replicates. Bar graph insets show fluorescence values normalized to t = 6 h. Graphs represent 3 biological replicates, error bars portray s.e.m.
