Figure 4: Overexpression of sAnk1 decreased glucose uptake in C2C12 cells.

(A) The efficiency of pEGFP-c1-sAnk1 transferred in C2C12 cells. C2C12 cells were transfected with the pEGFP-c1-sAnk1 plasmid. After differentiation, total RNA and protein isolates were analyzed by qPCR and western blotting, respectively. (B) Glucose uptake in C2C12 cells after transfection with the pEGFP-c1-sAnk1 plasmid and subsequent differentiation. The data shown are the mean ± S.D. P values were calculated by Student’s t-test.