Figure 3: MYH9 expression level correlates with PRRSV infection.
From: MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection
(a) TCID50 of PRRSV progeny virus titers in supernatant samples from cells with down-regulated MYH9 and western blot detection of MYH9 and PRRSV nuclear protein in the infected cells. The α-tubulin was tested as a loading control (left panel); TCID50 of pig rotavirus and western blot detection of MYH9 and pig rotavirus VP6 protein (right panel). (b) The effects of MYH9 overexpression on PRRSV (left panel) or pig rotavirus (right panel) replication in MARC-145 cells. (c) TCID50 of PRRSV progeny virus titers in supernatant samples from cells treated with MYH10 siRNA (siMYH9) or negative control siRNA (siNC) and Western blot detection of MYH9, MYH10 and PRRSV nuclear protein in the infected cells. (d) CD163 mRNA fold changes in MYH9 knockdown or overexpression cell lines, and in MYH10 knockdown cells. Error bars, mean ± s.e.m. (n = 3) *P < 0.05, **P < 0.01.
