Figure 4: MYH9 is essential for PRRSV infection.
From: MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection
(a) MARC-145, PK-15, PK-15CD163, COS-7, COS-7CD163, and COS-7CD163+MYH9 cell lines infected with PRRSV were fixed at 72 hrs post infection and imaged by confocal microscopy after staining with mouse antibodies against PRRSV N proteins (red). Nuclei (blue) were counter stained with DAPI. Scale bars, 50 μm. (b) TCID50 of progeny virus in the supernatant of the infected cells 72 hrs post infection. Error bars, mean ± s.e.m. (n = 3). *P < 0.05, **P < 0.01. (c) After allowing PRRSV bound to COS-7, COS-7CD163, COS-7CD163+MYH9, and MARC-145 cells at 4 °C for 1 hr, the cells were shifted to 37 °C for 0, 2, 4, 6 or 8 hrs. From the total mRNA, relative mRNA levels of PRRSV N gene were measured across the time course using quantitative RT-PCR. Relative folds of the N gene levels are shown in comparison with the N level in the COS-7CD163 cells at 37 °C for 2 hrs. Error bars, mean ± s.e.m. (n = 3).
