Figure 3: Compound 20 binds with MD2 via interacting with Arg90 and Tyr102. | Scientific Reports

Figure 3: Compound 20 binds with MD2 via interacting with Arg90 and Tyr102.

From: Discovery of new MD2 inhibitor from chalcone derivatives with anti-inflammatory effects in LPS-induced acute lung injury

Figure 3

(A) Molecular docking of compound 20 with MD2 protein was carried out with the Sybyl-2.0 molecular modeling software from Tripos. Compound 20 did not inhibit biotin-LPS binding to (B) rhMD2R90A/Y102A, (C) rhMD2R90A and (D) rhMD2Y102A determined by ELISA. SPR analysis showed no interaction between compound 20 and (E) rhMD2R90A/Y102A, (F) rhMD2R90A and (G) rhMD2Y102A respectively. Data are mean values (±SEM) of at least three separate experiments.

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