Table 3 Oligonucleotides used in this study.
Oligonucleotide | Sequence |
|---|---|
Oligonucleotides for isogenic deletion mutants | |
 essB-att1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGATGGCGACAGACAAAACCAAGC |
 essB-rev1 | CTATTTTTCCTCCTATAGTAACTTC |
 essB-rev2 | GTTACTATAGGAGGAAAAATAGGTATAGGACTGAGGCAAAGACAATGC |
 essB-att2 | GGGGACCACTTTGTACAAGAAAGCTGGGTGTGCCCATTTCAATGTTTCAAC |
 agr-att1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTACCCTTTCAATTGTCTGACG |
 agr -rev1 | GATGAATAATTAATTACTTTCATTGTAAA |
 agr-rev2 | AGTAATTAATTATTCATCACTTACCTATTTAACGTTTGTCTACA |
 agr-att2 | GGGGACCACTTTGTACAAGAAAGCTGGGTGGGATGCCTTTATTGGTG |
Oligonucleotides for genetic complementation | |
 EssB-Sma1-F | GAGCCCGGGAGATGGTTAAAAATCATAACCCTAAAAATG |
 EssB-BamH1-R | GAGGGATCCCTATTTTTTTCTTTCAGCTTCTT |
Oligonucleotides for qRT- PCR | |
 gyrB-F | CAAATGATCACAGCATTTGGTACAG |
 gyrB-R | CGGCATCAGTCATAATGACGAT |
 essB-F | TTTTCACAAGAGATGGATTACCAATT |
 essB-R | TTCTGACACCGGTAATGGATCA |
 esxA-F | CGTGCACAAGGTGAAATTG |
 esxA-R | CGAAACGGCTGAAAGCTT |
 essA-F | AAAAGCCCCTATATTCAAAACAA |
 essA-R | GCCCCTACAGACATCAAAATG |
 essC-F | TGCGTGCCTTAACATCAATC |
 essC-R | TAACGTCATGCTCTCCGAATAA |
 esaA-F | GTCAAGATGAACCAAATGATAAGAA |
 esaA-R | CCATCAAGGTTATGATTCAATGTT |
 esaB-F | CAAATTAAAGTGATGACGAAAGGT |
 esaB-R | CTCCATCAGCGATTTGATAATC |
Oligonucleotides for construction and purification of GST-tagged fusion protein | |
 ESSB-BamH1-F | GAGGGATCCATGGTTAAAAATCATAACCCTAAAAATG |
 ESSB-EcoR1-R | GAGGAATTCCTATTTTTTTCTTTCAGCTTCTT |
