Table 1 Km and Ki values (μM) of each NanA construct indicated the substrate affinity of NanA to MUNANA and its susceptibility to oseltamivir, respectively.

From: Sequence diversity of NanA manifests in distinct enzyme kinetics and inhibitor susceptibility

NanA

8919-LC

20566-LC

20566-CC

20566-CC-KGV

20566-CC-RAV

20566-CC-ΔKGI

9400-LC

9400-CC

K m a

24.40 ± 3.85

24.50 ± 2.71

25.97 ± 2.19

32.75 ± 4.78

17.92 ± 1.07

>1000

54.05 ± 9.11

51.27 ± 6.30

K i a

0.33 ± 0.02

0.22 ± 0.03

0.23 ± 0.03

0.12 ± 0.02

0.38 ± 0.04

—b

0.08 ± 0.02

0.07 ± 0.00

  1. aResults represent the mean ± s.d. from at least 3 independent determinations for each construct.
  2. bDue to the considerable activity reduction of the 20566-CC-ΔKGI, the Ki was not measured.
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