Figure 1 | Scientific Reports

Figure 1

From: The G protein-coupled receptor GPR157 regulates neuronal differentiation of radial glial progenitors through the Gq-IP3 pathway

Figure 1

GPR157 is expressed in primary cilia of RGPs in the developing neocortex.

(A) Expression of Gpr157 was analysed by RT-PCR using cDNAs derived from mouse neocorticies at E13 and P0. Nestin is a marker of RGPs. (B) E13 neocortical coronal sections immunostained with anti-GPR157 (green) and anti-ZO-1 (red) antibodies. Images of the entire cerebral wall are shown (upper panels). Magnified image of the boxed area in the upper panel was shown below. VZ, ventricular zone. CP, cortical plate. (C) High magnification images around the ventricular surface of E13 neocortical sections immunostained with anti-GPR157 (green) and anti-Ac-TUB (red) antibodies. Arrowheads indicate Ac-TUB-positive primary cilia protruding from the ventricular surface. (D) Left, Schematic diagram of en face view imaging. Right, High-magnification images of en face view of the ventricular surface of neocortices electroporated with either Lifeact-EGFP (upper panels) or Lifeact-EGFP/GPR157 shRNA (lower panels). The cortices were stained with antibodies against GPR157 and Ac-TUB. Closed and open arrowheads indicate primary cilia in transfected and non-transfected cells, respectively. LV: lateral ventricle, VZ: ventricular zone. Scale bars: 50 μm in (B) (upper panel); 10 μm in (B) (lower panel); 1 μm in (C,D).

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