Figure 3 | Scientific Reports

Figure 3

From: The G protein-coupled receptor GPR157 regulates neuronal differentiation of radial glial progenitors through the Gq-IP3 pathway

Figure 3

GPR157 couples with Gq-class of the heterotrimeric G-proteins.

(A–C) Plasmids expressing indicated protein were transfected into U-2 OS cells. Rhod4, a fluorescent calcium indicator, were used to assess changes in [Ca2+]i. Application of Ionomycin, a calcium ionopohore, to cells after experiments confirmed almost uniform uptake of Rhod4 in these cells. (D–F) Fluorescent intensity of Rhod4 in GFP-positive cells. Mean ± s.e.m. Data were obtained from 3 independent experiments (more than 40 cells). **p < 0.01, ***p < 0.001. Scale bar: 10 μm.

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