Figure 3

Far-UV CD spectra of WT and mutant MAGE-A4 proteins.
Spectra in the region of 260–195 nm were obtained at 6 °C, 20 °C, 37 °C, 90 °C, 37 °C after heating to 90 °C and at 20 °C after heating to 90 °C. (a) WT, (b) E138K, (c) P149S, (d) G153D, (e) E221K, (f) E224K, (g) E242K, (h) P267S, (i) R269C and (j) G316R.