Figure 4 | Scientific Reports

Figure 4

From: Consequences of point mutations in melanoma-associated antigen 4 (MAGE-A4) protein: Insights from structural and biophysical studies

Figure 4

Thermal unfolding curves of MAGE-A4 and its cancer-associated mutants recorded by DSF.

The data were recorded using MX3005P machine (Strategene) with the excitation and emission of SYPRO Orange dye set to be 470 nm and 570 nm, respectively. The proteins were diluted to 2 μM in 50 mM Tris buffer containing 150 mM NaCl, 5 mM DTT and 5% glycerol, pH 7.2.

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