Table 2 Generating FVII KO mice with gRNA and Cas9 mRNA co-injection.
From: Efficient generation of FVII gene knockout mice using CRISPR/Cas9 nuclease and truncated guided RNAs
ID gRNAs | No. injected embryos | No. embryos transferred | No. of Replicates | No. recipients | No. newborns | No. of newborns containing mutant allele(s)* | Mutant mice / total mice tested (%) (Mean ± SEM) | ||
|---|---|---|---|---|---|---|---|---|---|
Total No.mutant mice | No. with one allele (Alive) | No. with two alleles (Deceased) | |||||||
F7–1 | 78 | 71 | 3 | 3 | 18 | 1 | 1 (1) | 0 | 3.7 ± 3.7 a |
tF7–1 | 98 | 98 | 3 | 3 | 38 | 19 | 1 (1) | 18 (18) | 55.0 ± 5.0 b |
F7–2 | 110 | 106 | 3 | 4 | 24 | 8 | 2 (2) | 6 (6) | 35.8 ± 5.8 a |
tF7–2 | 75 | 67 | 3 | 3 | 20 | 15 | 15 (15) | 0 | 80.1 ± 7.4 b |
F7–3 | 80 | 77 | 3 | 3 | 6 | 2 | 2 (2) | 0 | 27.8 ± 14.7 a |
tF7–3 | 120 | 115 | 3 | 4 | 20 | 8 | 8 (8) | 0 | 39.4 ± 6.1 a |
