Figure 2: The expression levels of components of the ERAD pathway are altered in immune selected UKRV-Mel-15a cell clones. | Scientific Reports

Figure 2: The expression levels of components of the ERAD pathway are altered in immune selected UKRV-Mel-15a cell clones.

From: Exposure to Melan-A/MART-126-35 tumor epitope specific CD8+T cells reveals immune escape by affecting the ubiquitin-proteasome system (UPS)

Figure 2

(A) The UKRV-Mel-15a clones 6, 18 and 30 as well as the UKRV-Mel-15a parental cell line were subjected to protein extraction. Whole cell extracts were resolved by SDS-PAGE followed by Western blotting using antibodies specific for p97/VCP, VIMP, HERP, Melan-A/MART-1 and Derlin-1, as indicated. Equal protein loading was ensured by probing the membrane with anti-GAPDH antibody. (B) Fold changes of the expression levels of p97/VCP, HERP, VIMP and Derlin-1 between the UKRV-Mel-15a parental cell line and the UKRV-Mel-15a clones 6, 18 and 30, as visualized by densitometric analysis. The band intensity of each protein was normalized to that of GAPDH and subsequently expressed as fold-changes compared to the UKRV-Mel-15a parental cell line which was set at 1.

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