Figure 5: Melan-A/MART-126-35 epitope presentation in the immune challenged UKRV-Mel-15a clone 18 is not rescued by VIMP over-expression. | Scientific Reports

Figure 5: Melan-A/MART-126-35 epitope presentation in the immune challenged UKRV-Mel-15a clone 18 is not rescued by VIMP over-expression.

From: Exposure to Melan-A/MART-126-35 tumor epitope specific CD8+T cells reveals immune escape by affecting the ubiquitin-proteasome system (UPS)

Figure 5

(A) The UKRV-Mel-15a clone 18 was subjected to a 24-h transfection with an empty vector (mock) or expression vectors encoding VIMP, p97/VCP or p97QQ/VCP, as indicated. Whole cell-extracts were resolved on 15% SDS-PAGE and analysed by western-blotting using antibodies against VIMP, p97/VCP and β-actin (loading control). (B) The ability of the UKRV-Mel-15a clone 18 over-expressing VIMP, p97/VCP or p97QQ/VCP to present the Melan-A/MART-126-35 antigenic peptide was evaluated by exposing them to Melan-A/MART-126-35-specific CTL followed by IFN-γ measurement in the supernatants by ELISA. In addition, cells were pulsed with 1 μM of the Melan-A/MART-126-35 synthetic peptide, as a positive control. Shown is one representative experiment out of two. **p < 0.01 vs UKRV-Mel-15a clone 18 (mock) (Student’s t test).

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