Figure 3: CPEB1 competes with miR221/222 binding at an overlapping site in the 3′UTR of p27kip1. | Scientific Reports

Figure 3: CPEB1 competes with miR221/222 binding at an overlapping site in the 3′UTR of p27kip1.

From: CPEB1 restrains proliferation of Glioblastoma cells through the regulation of p27Kip1 mRNA translation

Figure 3

(A) p27 mRNA 3′UTR putative sites targeted by CPEB (orange) and by miR-221/222 seed sequence (green). (B) pUTRp27 or pUTRp27m1 were co-transfected with a plasmid expressing miR221/222 (p222/221) and pCPEB1 (last bar). After 48 h, HeLa cells were subjected to luciferase assay as in Fig. 2D. (C) pUTRp27 was co-transfected with pCPEB1 and/or LNA antisense oligonucleotides directed against endogenous miR-221 and miR-222 (antimiR). After 48 h, cells were treated as in (B). (D) HeLa cells were transfected with either pUTRp27 or pSeedMut together with pCPEB1 or an empty vector. After 48 h, cells were subjected to luciferase assay as in Fig. 2D. ANOVA test in combination with Dunnett’s test were performed to calculate p values. The mean of each column was compared with the mean of the control column.

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