Figure 2

Influence of Agr system and PSMα on colony spreading.

(A) S. aureus HG001, its agrD-deficient mutant, CGL005, psmα-deletion mutant, CGL007, CGL005(pGH-agr) and CGL007(pGH-psm) were cultured on 25 ml TSA-0.25 (a–g) and 25 ml TSA-0.4 (h–n) plates at 37 °C for 24 h. The mutants containing empty vector, i.e. CGL005(pGHL7) and CGL007(pGHL7), were used as controls. (B) Water (a,c,e,g) or 1 mM PSMα3 (b,d,f,h) was applied to the center of 25 ml TSA-0.25 (a,b,e,f) and 25 ml TSA-0.4 (c,d,g,h) plates. After drying for 30 min, 2 μl CGL005 (a–d) or CGL007 (e–h) was applied to the plates. Bacteria were cultured at 37 °C for 24 h.