Figure 4
From: Modulation of Staphylococcus aureus spreading by water
Height of colonies formed by S. aureus HG001, CGL005 and CGL007.
(A) A diagram depicts the side view (b) and top view (c) images acquired from Z stacks of the edge of the colonies with planes separated by 10 μm (a) under a confocal laser-scanning microscope. Green fluorescence indicates the distribution of bacteria in the colony. The plate contained carboxylate-modified FluoSphere beads and the last plane with red fluorescence indicates the position of medium surface. (B) TSA-0.4 plates were inoculated with PBS-washed S. aureus HG001(pRPO-gfp) (a), CGL005(pRPO-gfp) (b) and CGL007(pRPO-gfp) (c). After incubating at 37 °C for the time indicated, the top view images of Z stacks of the colonies were acquired under a confocal laser-scanning microscope. C: colony, M: medium.
