Figure 3
GW9508 attenuates LXR activation-induced triglyceride accumulation in HepG2 cells.
HepG2 cells were pretreated with either 50 or 100 μM GW9508 for 1 h and then treated with 5 μM T0901317 for 48 h. (A) Oil red O staining showed lipid droplets, bar = 25 μm. The data was representative of three independent experiments. Oil red O was then extracted by isopropanol and quantified with spectrophotometry at 520 nm. Cellular total triglyceride (B) and cholesterol (C) were measured. Data are expressed as means ± SEM and obtained from 3 individual experiments. **p < 0.01, compared with the control group; #p < 0.05, ##p < 0.01, ###p < 0.001, compared with T0901317-treated group.
