Figure 3
From: PATZ1 is a target of miR-29b that is induced by Ha-Ras oncogene in rat thyroid cells
Inhibition of proliferation in PATZ1-transfected FRTL5-Ras cells.
(a) Western blot analysis of PATZ1 in selected clones and mass populations (MP) of FRTL5-Ras-V29 cells transfected with PATZ1 (FR-PA) or the backbone vector (FR-BV). Asterisks indicate the mass populations and/or clones selected for the following experiments. (b) Growth curves on different stably expressing PATZ1 cell clones and/or mass populations of FRTL5-Ras cells (FRTL5-Ras-PATZ1) compared to controls expressing the empty vector (FRTL5-Ras-ctrl). Mean values ± SE of three clones or mass populations for each cell line are reported. FRTL5 and parental FRTL5-Ras-V29 (FRTL5-Ras) cells were also analysed for comparison of the results. (c) Flow cytometry (FACS) analysis on proliferating cells. Mean values ± SE of at least 3 independent experiments performed in two independent cell clones for each cell line are reported as percentages of the cell cycle distribution. *P < 0.05; **P < 0.01.
