Figure 4
From: PATZ1 is a target of miR-29b that is induced by Ha-Ras oncogene in rat thyroid cells
Inhibition of migration in PATZ1-transfected FRTL5-Ras cells.
(a) Representative images of a wound healing assay in control (FRTL-Ras-ctrl) and PATZ1-expressing FRTL5-Ras cells at 0, 24 h and 96 h after a confluent cell monolayer was wounded. (b) Percent of open wound, calculated as mean values ± SE of three independent cell clones or mass populations for each cell line, at 0 h, 24 h, 48 h, 72 h and 96 h after wound scratch. ***P < 0.001. (c) Representative images of a Transwell assay performed on FRTL5, FRTL5-Ras-ctrl and FRTL5-Ras-PATZ1 cells. Dark cells (stained with crystal violet) represent migrating cells. (d) Number of migrating cells expressed as mean values ± SE of three clones for each transfected construct. ***P < 0.001.
