Figure 4: Liposome sampling and size estimation.

Liposomes were sampled using a scanning band to which an unbiased counting rule was applied (a). All particles passing completely between the lines or intersecting the acceptance line (green) were selected for analysis. Particles intersecting the forbidden line (red) were excluded. Selected particles were translocated through size a systematic series of dots the upper edge of each tracing a scanning line. The particle caliper diameter was calculated from the number of intersection of dot edges with the nanoparticle and the real spacing of the dots as described in the text. Data are from three experimental runs from the same liposome preparation (b). Approximately 100 vesicles were counted per experiment; error bars are standard error of the mean (n = 3; each estimation took approximately 10 minutes).