Figure 5
From: Inhibiting DNA methylation switches adipogenesis to osteoblastogenesis by activating Wnt10a
Inhibition of DNA methylation by 5-Aza-dC promotes osteoblastogenesis in ST2 stem cells.
Treatment of 5-Aza-dC increases calcium content, measured by Alizarin Red staining (A) and quantitation (B) and alkaline phosphatase activity (C). ST2 cells were induced to differentiate into osteoblasts in the presence of either PBS or 5-Aza-dC at the time points indicated in the figures. Alizarin Red staining and alkaline phosphatase activity measurement were conducted as described in the Materials and Methods. Representative images are shown in (A). All data are expressed as mean ± SEM (n = 3–6); *p < 0.05 vs. control.
