Figure 6

Activation of the Wnt signaling mediates the anti-adipogenic effect of 5-Aza-dC in 3T3-L1 cells.

(A) Treatment of IWP-2, a Wnt inhibitor, largely prevents the inhibitory effect of 5-Aza-dC on the expression of adipogenic markers PPARγ, aP2, FAS and C/EBPα. (B) IWP-2 largely prevents the inhibitory effect of 5-Aza-dC on lipid accumulation measured by Oil Red O staining. 3T3-L1 cells were induced to differentiate and were concurrently treated with either PBS or 5-Aza-dC in the presence or absence of IWP-2 at day 1–2 of the differentiation period. Gene expression was measured by real-time RT-PCR and normalized to cyclophilin. Oil Red O staining was conducted as described in the Materials and Methods. Representative images are shown in (B). All data are expressed as mean ± SEM (n = 3–6); groups labeled with different letters are statistically different from each other.