Figure 7
From: Inhibiting DNA methylation switches adipogenesis to osteoblastogenesis by activating Wnt10a
Activation of the Wnt signaling mediates the osteoblastogenic effect of 5-Aza-dC in ST2 cells.
IWP-2 prevents the stimulatory effect of 5-Aza-dC on the expression of the osteoblastogenic marker BGLAP. ST2 stem cells were induced to differentiate with an osteoblastogenic regimen and were concurrently treated with either PBS or 5-Aza-dC in the presence or absence of IWP-2 in the differentiation period. ST2 cells were harvested at day 21 after fully differentiated into osteoblasts. Gene expression was measured by real-time RT-PCR and normalized to cyclophilin. All data are expressed as mean ± SEM (n = 3–6); groups labeled with different letters are statistically different from each other.
