Figure 3: The small RNome libraries of BSF and PCF.
(a) The pattern of the small RNAs present in post-ribosomal supernatant (PRS). Whole cell extracts from 109 PCF and BSF cells were prepared and depleted from ribosomes and described in “Methods” section. The RNA was extracted from the PRS and separated on a 6% denaturing gel and stained with ethidium bromide. T. Total RNA, 1. PCF and 2. BSF RNA from post-ribosomal supernatant. (b) Pie diagrams describing the RNA content of the small RNA libraries. Annotation of the reads obtained from RNA-seq of the small RNome PCF and BSF libraries. The percentage of the different RNA molecules among the reads are summarized in the pie chart. The percentage reported is the mean of the two replicates for each RNA class. (1) PCF library; (2) BSF library.
