Figure 4
(A) The first row (vertically): HepG2 cells were incubated with CPT (5 μM) for 40 min; The second row: HepG2 cells were incubated with 500 μM GSH and 250 μM Na2S2O3 for 1 h, and then with CPT (5 μM) for 40 min; The third row: HepG2 cells were incubated with 10 mM TNBS (2,4,6-trinitrobenzenesulphonate, known as a TST inhibitor) for 0.5 h, then with 500 μM GSH and 250 μM Na2S2O3 for another 1 h, followed by CPT (5 μM) for 40 min; The fourth row: HepG2 cells were incubated with 500 μM GSH for 1 h, then with CPT (5 μM) for another 40 min. (B) From left to right: the relative ratio of blue/red fluorescence intensity of row 1, 2, 3 and 4 in (A). The ratio images were all obtained as Fblue/Fred. Images were acquired from 405–555 nm for blue fluorescence, and from 560–700 nm for red fluorescence. λex = 405 nm.
