Figure 2

Detection of the LAMP products of C. sativus by using DNA electrophoresis (A) and calcein dyeing (B). The LAMP reaction was performed with the specifically designed primers. Lane B, lane N and lane T in (A) represent the blank lanes, the CK line (LAMP product without template DNA) and the positive amplification lane (LAMP product with template DNA), respectively. Lane M had 2 kb of DNA ladder marker; Tube N (without template DNA) and Tube T (with template DNA) in (B) represent the LAMP products stained with calcein dye.