Figure 3 | Scientific Reports

Figure 3

From: Rapid authentication of the precious herb saffron by loop-mediated isothermal amplification (LAMP) based on internal transcribed spacer 2 (ITS2) sequence

Figure 3

Specificity of the LAMP assay for the authentication of saffron and its adulterants.

(A) Turbidity was monitored with a Loopamp real-time turbidimeter at 400 nm every 6 s. (B) A visual colour change detection method was compared. 1 μl of calcein (a fluorescent detection reagent) was added to 25 μl of the LAMP reaction mixture before the LAMP reaction. Lines and tubes: 1, C. sativus 01; 2, C. sativus 11; 3, C. sativus 12; 4, C. sativus 13; 5, C. officinalis 01; 6, C. tinctorius 01; 7, C. longa 01; 8, D. carota 01; 9, N. nucifera 01; 10, Z. mays 01; and 11, Neg (negative control, ddH2O).

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