Figure 4 | Scientific Reports

Figure 4

From: Up-regulation of Interferon-inducible protein 16 contributes to psoriasis by modulating chemokine production in keratinocytes

Figure 4

IFI16 promotes CXCL10 and CCL20 expression via TBK1-NF-κB signaling pathway in keratinocytes.

(A) Immunofluorescence staining of NF-κB p65 (red) and phosphorylation of NF-κB p65 (p-p65) (Green) of human primary keratinocytes treated with IFI16 siRNA and cytokine cocktail, or transfected with IFI16 plasmid. Nuclei were counterstained with DAPI (blue). Bar, 20 μm. (B,C) The protein levels of NF-κB p65 and the phosphorylation of NF-κB p65 (p-p65). (D,E) mRNA levels of CXCL10 (D) and CCL20 (E). (F,G) Protein levels of CXCL10 (F) and CCL20 (G). Values represent mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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