Figure 5

Quantitative determination of protein and amino acid contents.
Protein content of WT was compared with that of COE (a), TOE (b) and untransformed plants regenerated from callus (c). The quantification was performed with five independent harvests. Total content of amino acids was evaluated in WT and transgenic lines by ninhydrin based calorimetric assay as detailed in ‘Materials and methods’ (d). Numbers on X-axis represent individual lines. Data represent mean ± s.e. of five measurements and asterisk (*) indicate the level of statistical significance at p < 0.05.