Figure 7 | Scientific Reports

Figure 7

From: Epigenetically regulated miR-449a enhances hepatitis B virus replication by targeting cAMP-responsive element binding protein 5 and modulating hepatocytes phenotype

Figure 7

Regulation of HBV replication and transcription, FXRα and ALB expression and cell cycle progression by HCC-related miRNAs.

HepG2.2.15 cells were transfected with different miRNA mimics at 20 nM and then cultured for 4 days. (A) HBV replication and transcription were determined by Southern blotting (upper panel) and real-time RT-PCR (bottom panel), respectively. (B) FXRα and ALB expression was determined by western blotting. (C) miRNA-transfected HepG2.2.15 cells were treated with nocodazole (100 nM) for 24 hours and the distribution of cells in cell cycle phases G1, S and G2/M was assessed by flow cytometry using propidium iodide staining. *P < 0.05.

Back to article page