Figure 6: Nox4 regulates ROS generation and flagellin-induced pro-inflammatory mediator production in ApoE knockout mice.
From: Flagellin-induced NADPH oxidase 4 activation is involved in atherosclerosis
(A) Expression of a pro-inflammatory mediator was assayed by real-time PCR using endothelial enriched RNA obtained from the thoracic aorta after 2 hrs of i.v. injection with saline or rFliC (25 μg/mouse). Endothelial cell purity was qualified in each RNA (SMA/PECAM-1 <0.001). Quantification of the ICAM-1 mRNA expression, ***p < 0.001. Quantification of the VCAM-1 mRNA expression, **p < 0.01, ***p < 0.001. Quantification of the MIP-2 mRNA expression, **p < 0.01, ***p < 0.001. Quantification of KC mRNA expression, ***p < 0.001. (N = 3, mean ± SEM). (B) Thoracic aorta sections of ApoE KO and Nox4ApoE DKO mice were stained with 4-hydroxy-2-nonenal (4-HNE) for detection of oxidative stress-dependent lipid peroxidation. L means lumen. Scale bar: 50 μm. (C) Quantification of fluorescence intensity of 4-HNE staining using image J. (N = 3, mean ± SEM, ***p < 0.001).
