Figure 8: Analysis of atherosclerosis plaque composition in ApoE KO or Nox4ApoE dKO mice.
From: Flagellin-induced NADPH oxidase 4 activation is involved in atherosclerosis
(A) Sinus sections were obtained from ApoE KO or Nox4ApoE DKO mice fed high-fat diet for 12 weeks with or without rFliC injection. Each samples was stained with anti-monocyte/macrophage antibody (MOMA-2, AbD Serotec). (B) Quantification of MOMA-2 positive area in sinus sections from ApoE KO or Nox4ApoE DKO mice (N = 5 to 10, mean ± SEM, *p < 0.05, **p < 0.01). (C) Quantification of MOMA-2 positive area in total lesion area, N = 5 to 10, mean ± SEM, *p < 0.05, ***p < 0.001. (D) Sinus sections were stained with anti-neutrophil antibody (Abcam). (E) Quantification of neutrophil positive area in sinus sections from ApoE KO or Nox4ApoE DKO mice using image J (N = 5 to 10, mean ± SEM, ***p < 0.001). (F) Quantification of neutrophil positive area in total lesion area, N = 5 to 10, mean ± SEM, *p < 0.05, ***p < 0.001. (G) Sinus sections were stained with anti-smooth muscle actin (Dako). (H) Quantitative analysis of anti-smooth muscle actin positive area, N = 5 to 10, mean ± SEM, ***p < 0.001. (I) Quantification of smooth muscle actin positive area in total lesion area, N = 5 to 10, mean ± SEM, *p < 0.05, ***p < 0.001.
