Figure 2 | Scientific Reports

Figure 2

From: Deregulated hedgehog pathway signaling is inhibited by the smoothened antagonist LDE225 (Sonidegib) in chronic phase chronic myeloid leukaemia

Figure 2

The Hh pathway is expressed at the protein level in human haemopoietic cells with the machinery (i.e. primary cilia) to enable canonical Hh signaling.

(A) Percentage of SHH, DHH and IHH positive cells per bone marrow trephine (BMT) specimen in CP-CML (n = 7) compared to normal (n = 5). Results represent the mean ± SEM for multiple samples. Significance values; *p < 0.05. (B) Examples of BM trephine staining of SHH by immunohistochemistry. (C) Example of confocal images of primary cilia from BM trephine samples comparing CP-CML (n = 10) to normal controls (n = 10). There was no significant difference in the number of primary cilia observed between normal and CP-CML BM haemopoietic cells. Scale bar 100 μM in all images. Arrows highlight primary cilia demonstrated by co-localisation of α- and γ-tubulin.

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