Figure 5: LPS-induced levels of IL-1β are inhibited by LXR activation.
From: The nuclear receptor LXR modulates interleukin-18 levels in macrophages through multiple mechanisms
(a) BMDM were treated with vehicle (DMSO), GW3965 (1 μ mol/L) or LXR antagonist (LXR ant) for 24 hours with or without LPS (100 ng/ml) for the last 6 hours. IL-1β mRNA levels were analyzed by RT-qPCR. Values indicate expression normalized to cyclophilin and are presented relative to expression in LPS-treated cells, set as 1. Values represent the mean ± SEM (n = 3–4). t-test: ***p < 0.001, ns p > 0.05. (b) Secreted IL-1β levels in cell supernatants were quantified by ELISA. Values represent the mean ± SEM (n = 3) **p < 0.01.
