Figure 4 | Scientific Reports

Figure 4

From: Convergent Transcription of Interferon-stimulated Genes by TNF-α and IFN-α Augments Antiviral Activity against HCV and HEV

Figure 4

TNF-α activates ISRE via TNF receptor I.

(A) qRT-PCR analysis of TNFR1 knockdown by lentiviral shRNA vectors in the Huh7 based ISRE luciferase reporter cells. Compared to the control vector transduced cells, the two shRNA treated clones (sh-02 and sh-03) showed strong reduction of TNFR1 RNA levels. (B) TNFR1 knockdown had no significant influence on IFN-α induced ISRE-related luciferase activation as measured at 3 different time points (n =  3 independent experiments with 2–3 replicates each). (C) TNFR1 knockdown blocked TNF-α induced ISRE-related luciferase activation as measured at 3 different time points (n =  3 independent experiments with 2–3 replicates each).

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